This enzyme is used for enzymatic determination of triglyceride in serum when coupled with L-α-glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311). Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5～3.0 units of the enzyme per test (3.0ml) at pH around 7.0.
Product name: Triacylglycero-protein acylhydrolase
Appearance: Light brown amorphous powder lyophilized
Activity: Grade III, 20U/mg-solid or more (containing approx. 80% of stabilizers)
Contaminants: NADH oxidase ≤ 1.0×10-3 %
Phosphatase ≤ 1.0×10-3 %
Catalase ≤ 2.0×10-2%
Cholesterol oxidase ≤ 2.0×10-3 %
Stabilizers: Mg2+, Na-cholate, bovine serum albumin
Stability: Stable at －20°C for at least one year
Molecular weight: approx. 134,000
Isoelectric point: 5.95 ± 0.05
Inhibitors: Hg2+, Ag+, ionic detergents
Optimum pH: 7.0－9.0
Optimum temperature: 45－50°C
pH Stability: pH 7.0－9.0 (25°C, 20hr)
Thermal stability: below 55°C (pH 7.0, 10min)
EC # 3.1.1.34