Product name: ATP: Glycerol 3-phosphotransferase
Appearance: White amorphous powder lyophilized
Activity: Grade Ⅲ 30 U/mg-solid or more
Contaminants: Catalase : ≤ 1.0×10-1 %, NADH oxidase : ≤ 1.0×10-3 %
Adenosine triphosphate : ≤ 1.0×10-3 %
Stability: Stable at -20°C for at least 12 months
Molecular weight: approx. 220,000 (by gel filtration)
Structure: 4 subunits of approx 58,000
Isoelectric poin:t 4.3
Michaelis constants: 9.4×10-5 M (Glycerol), 1.3×10-5 M (ATP), 2.1×10-3 M (dihydroxiacetone)
Inhibitors: p-Chloromercuribenzoate, Hg++, Ag+
Optimum pH: 10.0
Optimum temperature: 70℃
pH Stability: pH 5.5 – 10.0 (25°C, 20hr)
Thermal stability: below 65℃ (pH 7.5, 30min)