Product name: (S)-Lactate: NAD+ oxidoreductase
Appearance: Crystalline suspension in 1.6M ammonium sulfate solution
Activity: GradeⅡ350U/mg-protein or more
Contaminants: Malate dehydrogenase ≤ 2.0×10-2%
Myokinase ≤ 1.0×10-2%
Pyruvate kinase ≤ 2.0×10-3%
Stability: Stable at 5℃ for at least one year
Molecular weight: 115,000±6,500
Michaelis constants: 2.5×10-2M (Lactate), 1.0×10-2M (Pyruvate)
Structure: 4 subunits per mol of enzyme, E1%1cm at 280 nm＝11.52 (Biuret method)
Inhibitors: I－, Ag＋, Hg2＋, p-chloromercuribenzoate, LDH inhibitors (formed from NADH)
Optimum pH: 6.0－7.4
Optimum temperature: above 60℃
pH Stability: pH 6.0－8.0(23℃, 22hr)
Thermal stability: below 50℃(pH 7.4, 10min)
EC # 1.1.1.27
Used in In Vitro Diagnostic tests and Life Sciences, Research & Development
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