Molecular Weight: 75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G150)
Isoeletric Point: ~4.2 (230 000 Da (Sephadex G 200), 4 x 58 000 Da (SDS-gel electrophoresis))
Michaelis Constan (L-glycerol phosphate):
K-phosphate buffer, 0.1 mol/L; pH 7.5: 1.36 x 10-2 mol/L (ensaio o-dianisidine)
Tris 0.1 mol/L; pH 7.6: 2.90 x 10-3 mol/L (o-dianisidine assay)
Tris 0.1 mol/L; pH 8.1: 1.40 x 10-3 mol/L (PAP assay)
Structure: Monomeric with FAD as cofactor
Inhibitors: Ag, Hg salts and SDS
pH optimum: 8.0-8.5
