Product name: L-Leucine: NAD+ oxidoreductase (deaminating)
Appearance: White amorphous powder lyophilized
Activity: Grade Ⅱ, 20U/mg-solid or more (containing approx. 70% of stabilizers)
Contaminants: Leucylpeptide decomposing enzymes :
(Leu-Val) ≤ 1.0×10-2%
(Leu-Gly-Gly) ≤ 1.0×10-2%
NADH oxidase ≤ 1.0×10-2%
Stabilizers: 2-Mercaptoethanol, L-cysteine, dithiothreitol, ethylenediaminetetraacetate
Stability: Stable at －20℃ for at least one year
Molecular weight: 245,000
Michaelis constants: 1.0×10-3M (L-Leucine), 3.9×10-4M (NAD＋), 3.5×10-5M, (NADH), 3.1×10-4M［α-Ketoisocaproate (α-K Ⅰ C)］, 2.0×10-1M (NH3)
Structure: 6 subunits per mol of enzyme
Inhibitors: Na2S, Hg2+, Cu2+, Co2+, Mg2+, p-chloromercuribenzoate
Optimum pH: 10.5－10.8 (L-Leu→α-KⅠC), 9.4 (α-K Ⅰ C→L-Leu )
Optimum temperature: Above 70℃
pH Stability: pH 5.5－10.5 (25℃, 20hr)
Thermal stability: Below 60℃ (pH 6.9, 10min)
EC # 1.4.1.9