Name: Urea amidohydrolase
Appearance: white amorphous powder lyophilized
Activity: Grade II, 100U/mg-solid or more
Contaminants: Asparaginase ≤ 0.02 %
Arginase ≤ 0.002 %
NH4+ ≤ 5.0×10-4 μg/U
Stability: Stable at －20℃ for at least 12 months
Molecular weight: approx. 480,000
Isoelectric point: 5.0 – 5.1
Michaelis constant: 1.05×10-2 M (Urea)
Structure: 8 active sites with SH-groups per mole of the enzyme
Inhibitors: Heavy metal ions (Ag+ Hg2+ etc.)
Optimum pH: 6.0
Optimum temperature: 60°C
pH Stability: pH 5.5 – 8.5 (30°C, 17hr)
Thermal stability: below 50°C (pH 8.0, 60min)
EC # 3.5.1.5