Product name: L-malate: NAD+ oxidoreductase
Appearance: Slightly yellowish amorphous powder, lyophilized
Activity: Grade II 40U/mg-solid or more
Contaminants: Glutamate oxaloacetate transaminase ≤ 1.0×10-3 %
Lactate dehydrogenase ≤ 1.0×10-3 %
NADH oxidase ≤ 1.0×10-3 %
Stability: Stable at – 20°C for at least 12 months
Molecular weight: Approx. 140,000
Isoelectric point: pH 4.8 ± 0.1
Michaelis constants: 5.4×10-5M (L-Malate), 5.0×10-6 M (Oxaloacetate), 8.1×10-6M
(NADH)
Structure: 4 subunits per mole of enzyme
Inhibitors: Hg2+
Optimum pH: 8.0
Optimum temperature: 70°C
pH Stability: pH 3.0 – 9.0 (25°C, 20hr)
Thermal stability: Below 70°C (pH 7.5, 15min)
EC # 1.1.1.37