Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
High activity and exceptional purity.
Active at high temperatures (up to 56 °C) and under denaturing conditions (e.g. in the presence of urea and/or SDS), which makes it ideal for digesting proteins in a variety of applications.
Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0).
Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
Available as powder, lyophilized “cake” or liquid. Solubility in water ≥ 20 mg/ml Unit definition One unit of Proteinase K hydrolyzes urea-denatured hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU. Stability: Proteinase K Lyophilized powder maintains activity ≥ 30 U/mg and specific activity ≥ 40 U/mg for at least 24 months, stored in its original, unopened container. Appearance White lyophilized powder Solubility in water ≥ 20 mg/ml Activity 1 ≥ 30 U/mg lyophilizate Specific activity 1 ≥ 40 U/mg protein Protein content 2 ≥ 70% DNA content 3 ≤ 10 pg/mg Exonuclease activity 4 None detected Endonuclease activity 5 None detected RNase activity 6 None detected