Product name: Glycerol:NAD+ 2-oxydoreductase
Appearance: White amorphous powder lyophilized
Activity: Grade III 50 U/mg-solid or more (containing 50% of stabilizers)
Contaminants: NADH oxidase ≤ 1.0×10-3 %
Stabilizers: BSA
Stability: Stable at －20°C for at least 12 month
Molecular weight: approx. 390000
Isoelectric point: 4.4±0.1
Michaelis constants: 1.1×10－2M (Glycerol) 8.9×10－5M (NAD＋)
Structure: 10 subunits (42000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions (Co2+, Ni2+, Cu2+, Zn2+, Cd2+)
Optimum pH: 10.0－10.5
Optimum temperature: 50°C
pH Stability: pH 7.5－10.5 (25°C, 20hr)
Thermal stability: below 55°C (pH 7.5, 15min)
Substrate specificity: This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene glycol and 2,3-butanediol. The oxidative reaction is stimulated by K+, NH4+ and Rb+.
EC # 1.1.1.6