Name: Purine-nucleoside: orthophosphate ribosyltransferase
Appearance: White amorphous powder lyophilized
Activity: Grade III, 15 U/mg-solid or more
Contaminants: Catalase ≤ 20%
5′-Nucleosidase ≤ 1.0×10-3 %
Adenosine deaminase ≤ 1.0×10-3 %
ATPase ≤ 1.0×10-2 %
Stabilizers: K-Gluconate, mannitol, EDTA
Stability: Stable at – 20℃ for at least 12 months
Molecular weight: Approx. 120,000
Isoelectric point: 4.1±0.1
Michaelis constants: 6.4×10-5 M (Inosine), 3.2×10-4 M (Pi)
Inhibitors: p-Chloromercuribenzoate, SDS, Hg2+, Ag+
Optimum pH: 7.5－8.0
Optimum temperature: 65℃
pH Stability: pH 6.0－9.0 (30℃, 16hr)
Thermal stability: Below 60℃ (pH 7.7, 30min)
EC # 2.4.2.1