Molecular weight: 57 kD (SDS-PAGE)
Isoelectric point: 4.5-5.0
Michaelis constants (D-glucose):
Phosphate buffer, 0.1 mol/L, pH 7.0; +25°C: 3.05 x 10-4 mol/L
Phosphate buffer, 0.1 mol/L, pH 7.4; +30°C: 1.90 x 10-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 2.30 x 10-4 mol/L
Michaelis constants (ATP):
Tris buffer, 0.1 mol/L, pH 7.6; +28°C: 1.60 x 10-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 1.90 x 10-4 mol/L
Inhibitors: EDTA, SH-blocking compounds, sorbose-1-phosphate,
polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxymethylglucose,
lyxose.
Activators: Mg2+, catecholamines
pH optimum: 7.0-10.0 (see figure for product Cat. No. 11 119 796 103)
Temperature dependence: See figure for product Cat. No. 11 119 796
103
pH stability: 5.0-9.0 (see figure for product Cat. No. 11 119 796 103)
Thermal stability: See figure for product Cat. No. 11 119 796 103
Solubility: Clear, colorless solution in water (c=40 mg/mL)
pH value (c=40 mg/mL in water): 6.5-7.5
Activity (+25°C, glucose): ≥40 U/mg lyophilizate
Stability: At +2 to +8°C within specification range for 18 months.
Remark: This enzyme is especially suited for liquid stable applications
with extended shelf life requirements.