Name: Urea amidohydrolase
Appearance: white amorphous powder lyophilized
Activity: 220U/mg-solid or more (Nessler units)
Contaminants: NH4+ ≤ 2.0×10-4 μmol/U
Stability: Stable at -20°C for at least 6 months
Molecular weight: approx. 480 000
Isoelectric point: 5.0—5.1
Michaelis constant: 1.05×10-2 M (Urea)
Structure: 8 active sites with SH-groups per mole of the enzyme
Inhibitors: Heavy metal ions (Ag+ Hg2+ etc.)
Optimum pH: 6.0
Optimum temperature: 60°C
pH Stability: pH 5.5—8.5 (30°C, 17hr)
Thermal stability: below 50°C, (pH 8.0, 60min)
EC # 3.5.1.5