Name: Sarcosine: oxygen oxidoreductase (demethylating)
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III, 8U/mg-solid or more
Contaminants: Catalase ≤ 1%
Stabilizers: Potassium chloride
Stability: Stable at – 20 °C for at least one year
Molecular weight: Approx. 43,000 (by SDS-PAGE)
Isoelectric point: 4.8 ± 0.1
Michaelis constants: 2.8×10-3 M
Inhibitors: Cu2+, Ag+, Hg2+, p-chloromercuribenzoate, N-ethylmaleimide, SDS
Optimum pH: 7.5 – 8.5
Optimum temperature: 55 – 60 °C
pH Stability: pH 6.0 – 9.5 (25 °C, 20hr)
Thermal stability: below 60 °C (pH 7.5, 10min)
EC # 1.5.3.1