Product name: L-Glutamate:NADP+ oxydoreductase (deaminating)
Appearance: 50mM Tris buffer solution at pH 7.8 with 0.05% NaN3 and 5mM EDTA
Activity: > 9000 U/ml
Contaminants: NADPH oxidase ≤ 0.01 %, Gluthathione reductase ≤ 0.01 %
Stabilizer: EDTA
Stability: Stable at 2-8°C for at least 6 months
Molecular weight: Approx. 300,000
Isoelectric point: 4.6
Michaelis constants: 1.1×10-3 M (NH3), 3.4×10-4 M (α-Ketoglutarate), 1.5×10-5M (NADP+)
1.2×10-3 M (L-Glutamate), 1.4×10-5M (NADPH)
Structure: 6 subunits per mol of enzyme
Inhibitors: Heavy metals, PCMB, Pyridine, 4-4’-dithiopyridine, 2-2’-dithiopyridine
Optimum pH: 8.5 (α-KG→L-Glu), 9.8 (L-Glu→α-KG)
Optimum temperature: 45°C (α-KG→L-Glu), 45-55°C (L-Glu→α-KG)
pH stability: 6.0 – 8.5 (25°C, 20hr)
Thermal stability: Below 50°C (pH 7.4, 10min)
EC # 1.4.1.4
Used in In Vitro Diagnostic tests and Life Sciences, Research & Development
2-8º