Nomenclature: D-Biotinyl-ε-amido caproic acid N-hydroxysuccinimid
ester
Formula: C20H30N4O6S
Molecular weight: 454.5 D
Remark: Under mild conditions the activated ester reacts with amino
groups. The aminocaproic acid spacer is useful if biotinylated
macromolecules are coupled because steric hindrance is minimized. Appearance: White to beige powder
Biotin ester (from N): ≥97-103%
N (elementary analysis): 11.9-12.8%
C (elementary analysis): 51.0-54.7%
H (elementary analysis): 6.4-6.9%
Purity (TLC: silica gel, 1-butanol/glacial acetic acid/H2O= 50/15/25,
iodide stream/UV): Chromatographically homogeneous
Hydrolysis product (NMR): ≤20%
Stability: At -15 to -25°C within specification range for 24 months