This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201, URH-301) in clinical analysis.
Product name: L-Glutamate:NAD+ oxydoreductase (deaminating)
Appearance: White amorphous powder lyophilized
Activity: Grade II, 100 U/mg-solid or more
Contaminants: NAD oxidase < 1.0×10-2 %
Stability: Stable at – 20 °C for at least one year
Molecular weight: approx. 260,000
Isoelectric point: 5.6
Michaelis constants: 9.21×10-3 M (NH3), 4.80×10-3M (α-Ketoglutarate),
5.89×10-4M(NAD＋) 7.8×10-5M (L-Glutamate), 1.29×10-4M (NADH)
Structure: 6 subunits per mol of enzyme
Inhibitors: Heavy metals, PCMB, IAA
Optimum pH: 7.5 – 8.0 (α-KG→L-Glu), 9.0 (L-Glu→α-KG)
Optimum temperature: 55 °C (α-KG→L-Glu), 50 °C (L-Glu→α-KG)
pH Stability: pH 5.0 – 10.0 (25 °C, 20hr)
Thermal stability: below 50 °C (pH 8.3 ,10min)
EC # 1.4.1.2