Name: L-Alanine:2-oxoglutarate aminotransaminase. ALT/ALAT.
Appearance: Yellow-green powder lyophilized
Activity: 70 u/mg-solid or more at 37°C
Contaminants: CPK < 8×10-4 %, AST (GOT) < 0.1 %, LDH < 8×10-3 %
GTD < 10-2 %, Protease < 2×10-3 %, Ammonia < 10-3 %
Storage: Store at -20°C
Molecular weight: approx. 100 000
Unit definition : One unit will catalyze the transamination of 1 micromole of LAlanine and alpha-ketoglutarate,
forming L-glutamate and pyruvate per minute at 37°C and pH 7.8. Measured at 340nm as 1 equimolar amount
of NAD produced by a coupled reaction.
Expiration: Stable at least 1 year at –20°C
EC # 2.6.1.2