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D-3-Hydroxybutyrate dehydrogenase from Pseudomonas sp

(R)-3-hydroxibutanoate: NAD+ oxidoreductase. EC # 1.1.1.30

Descrição do Produto:

Product name: (R)-3-hydroxibutanoate: NAD+ oxidoreductase
Appearance: white amorphous powder lyophilized
Activity: Grade III 100 U/mg-solid or more
Stabilizers: Sucrose, manitol, BSA
Stability: Stable at －20°C for at least one year
Molecular weight: approx. 130,000 (by gel filtration)
Isoelectric point: 5.6 ± 0.1
Michaelis constants: 4.2×10-4M (25°C, pH8.3), 7.0×10-4M (37°C, pH8.3) (D-3-Hydroxybutyrate)
4.9×10-5M (25°C, pH8.3), 7.2×10-5M (37°C, pH8.3) (NAD+)
8.1×10-5M (25°C, pH7.1), 2.4×10-4M (37°C, pH7.1) (Acetoacetate)
8.4×10-6M (25°C, pH7.1), 1.5×10-5M (37°C, pH7.1) (NADH)
Inhibitors: PCMB, MIA, IAA, Ag+, Hg2+, SDS, DAC
Optimum pH: 8.3
Optimum temperature: 55°C
pH Stability: pH 5.0－8.5 (25°C, 20hr)
Thermal stability: below 40°C (pH 6.5, 15min)

Aplicação:

Used in In Vitro Diagnostic tests and Life Sciences, Research & Development

D-3-hydroxybutyrate + NAD + —-> Acetoacetate + NADH + H+ D-3-hydroxybutyrate + NAD + <—- Acetoacetate + NADH + H+ This enzyme is used for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.

Armazenamento:

Armazenar a < -20ºC

Apresentações:

5 KU

25 KU

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> 100 KU

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Orçamento Enzytec Produtos

D-3-Hydroxybutyrate dehydrogenase from Pseudomonas sp