Appearance: Yellowish amorphous powder lyophilized
Activity: Grade III 30U/mg-solid or more (containing approx. 15% of stabilizers)
Contaminants: Myokinase ≤ 5.0×10-1%
NAD(P)H oxidase ≤ 5.0×10-1%
Stabilizers: FMN, NAD(P)H
Stability: Stable at -20 °C for at least one year
Molecular weight: 24,000
Michaelis constants: 2.0×10-5 M (NADH), 6.0×10-6 M (NADPH)
Structure: One mol of FMN per mol of enzyme
Inhibitor: N-Ethylmaleimide
Optimum pH: 8.5
Optimum temperature: 50 °C
pH Stability: pH 7.5 (30 °C, 3hr)
Thermal stability: below 30 °C (pH 7.5, 30min)
Substrate specificity: Either NADH or NADPH can be used as a reductant. The ratio (NADPH/NADH) is 0.6 in the assay method. Neither oxygen nor cytochrome C can be utilized as a hydrogen acceptor.
Used in In Vitro Diagnostic tests and Life Sciences, Research & Development
Diaphorase from Clostridium NAD(P)H + H+ + Acceptor (ox) —-> NAD(P)+ + Acceptor (red) This enzyme is used for colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.
< -20ºC