Formula: C4H6NOSCl. 2-methyl-3(2 H)-isothiazolonne-hydrochloride. Molecular Weight: 151.62 D (MIT: 115.12 D). Antimicrobial effect: Highly effective agains bacteria, yeast and fungi. Possible combination: Can be well combined with all preservatives. Combination with preservatives that have a good effect against fungi (e.g., 5-bromo-5-nitro-1,3-dioxan (BND), 2-hydroxypridine-N-oxide (HPO)) results in a very broad spectrum of effectiveness. Effect: Tought to react with -NH2 and -SH groups of all proteins. Interferences: Probably possible with amines and sulfides. Toxicity: Harmless at recommended concentrations; not carcinogenic or mutagenic (Ames-test negative). Recommended working concentration: 0.4 mg/ml, if both risk of contamination and concentration of protein are low (< 0.2%); 1.0 mg/ml, if both the risk of contamination and concentration are high. pH optimum: 4.0-7.0. Stability: Decreases at pH > 8.5 and > + 50°C; no loss of activity when hetaed up to + 100°C for a short period os time. Appearance: White to light beige crystalline powder. Solubility: Clear, colourless solution in water (c = 10 mg/ml), free from fuzz. A405 (c = 10 mg/ml water, against water): ≤ 0.025. Melting range (Büchi): + 160 to + 180°C. Identity (NIR): Corresponds to reference. N-Methylisothiazolone (GC): ≥ 99 area%. Water (K. Fischer): ≤ 0.5%. Impurities (GC): ≤ 1 area %. Heavy metals (as Pb): ≤10 ppm. Oxidizing substances: ≤ 2 %. Acetone (GC) : ≤ 0.2 %. 2-Propanol (GC): ≤ 0.2 %. Petrolether (GC): Beyond the limit of detection (approximatly 1%); Corresponds to reference. Elecsys Hbs AG: Corresponds Stability: At + 2 to + 8°C within specification range for 24 months.