Formula: C12H12N3O7NH4
Molecular weight: 328.3 D
Appearance: White to yellowish crystalline powder
Solubility: Clear, yellow solution in water (c=100 mg/mL), free of fuzz
pH value: 4.0-6.0
Molar rotation [α] 25/D: +32.0±2.0°
Melting range (Kofler): Approximately +170 to +180°C
Glupa-carboxylate, free acid (enzymatic): ≥87%
Glupa-carboxylate (HPLC): ≥99 area%
Water (K. Fischer): ≤6.2%
NH4 (Neßler’s reagent): 5.2±1% 5-Amino-2-nitrobenzoate (HPLC): ≤0.1 area%
α-Glupa-carboxylate (HPLC): ≤0.4 area%
Thin layer chromatography (silica gel F; n-butanol/glacial acetic acid/
H2O = 50/15/25; UV, with Nihydrin): Chromatographically homogeneous
A405 (Glupa-carboxylate, 6 mmol/L): 0.65-0.80
Stability: At +2 to +8°C within specification range for 24 months.
Protect from light.