Molecular weight: 230 000 D (Sephadex G 200), 4 x 58 000 D (SDS-gel
electrophoresis)
Michaelis constants (Glycine buffer, pH 9.8; +30°C):
Glycerol: 4.4 x 10-5 mol/L
Inhibitors: Unknown; Inhibitors of glycerokinase from Candida
mycoderma do not inhibit the glycerokinase from Bacillus
stearothermophilus.
pH optimum: 10.0-10.5 (see figure for product Cat. No. 11 499 530 103)
Temperature dependence: See figure for product Cat. No. 11 499 530
103
pH stability: 5.0-11.0 (see figure for product Cat. No. 11 499 530 103)
Thermal stability: Up to +60° C (see figure for product Cat. No. 11 499
530 103). Appearance: Clear, colorless to slightly yellowish solution in Tris buffer;
pH approximately 7.3, stabilized, potential particles as result of
recristallized salts
pH value (c=10 mg/mL, in water): 7.1-7.5
Activity (+25°C, glycerol): ≥2,200 U/mL
Specific activity: ≥85 U/mg protein
Protein (Biuret): No limit
Stability: At +2 to +8°C within specification range for 12 months