Nomenclature: D-Biotinyl-N-hydroxy-succinimide ester
Formula: C14H19N3O5S
Molecular weight: 341.4 D
Remark: Under mild conditions the activated ester reacts with amino
groups. The aminocaproic acid spacer is useful if biotinylated
macromolecules are coupled because steric hindrance is minimized. Gel
suspension (50%), ready to use, bulk format.
Specification
Appearance: White crystallizate
Biotin ester (from N): 98-103%
N (elementary analysis): 12.0-12.73%
Purity (TLC: silica gel, 1-butanol/glacial acetic acid/H2O= 2/1/1; a) in
UV; b) with 4-Dimethylamino cinnamaldehyde): Chromatographically
homogeneous
Stability: At +2 to +8°C within specification range for 24 months.
Use D-Biotin-N-hydroxysuccinimide ester as biotinylating reagent for
proteins and amino labeled oligonucleotides.
Used in In Vitro Diagnostic tests and Life Sciences, Research & Development
< -20ºC