Product name: Prolyl endopeptidase
Appearance: White amorphous powder lyophilized
Contaminants: Trypsin-like activity ≤ 0.1%
Leucine aminopeptidase ≤ 0.1%
Activity: Grade I, 5.0 U/mg-solid or more
Stability: Stable at – 20℃ for at least 12 months
Molecular weight: Approx. 78,000
Isoelectric point: 9.1
Michaelis constants: 2.5×10-5 M (Z-Gly-Pro-MCA), 1.4×10-4 M (Z-Gly-Pro-2NNap)
Structure: Monomer
Inhibitors: DFP, 3, 4-dichloroisocoumarin, Z-Gly-Pro-CH2Cl
Optimum pH: 6.5
Optimum temperature: 37℃ (40℃)
pH Stability: 5.5－8.5 (30℃,15hr)
Thermal stability: below 40℃ (pH7.0,10min)
Substrate specificity: Y-Pro(Ala)-X (Y, peptide or N-protected amino acid; X, amino acid, peptide, amide, or ester)
EC# 3.4.21.26