β-D-glucoside glucohydrolase
EC 3.2.1.21
Appearance Light yellow amorphous powder lyophilized
Activity Grade II, 10 U/mg-solid or more (containing approx. 50% of BSA)
Contaminant α-Amylase ≤ 5.0×10-4 %
Stabilizers Bovine serum albumin (BSA), glutathione (reduced)
Stability Stable at －20℃ for at least 12 months
Molecular weight approx. 110,000
Isoelectric point 7.3
Michaelis constants 2.8×10-3 M (p-Nitrophenyl-β-D-glucopyranoside), 3.3×10-3 M (2,4
Dichlorophenyl-β-D-glucopyranoside)
Structure: 2 subunits per mol of enzyme
Optimum pH 5.5
Optimum temperature 50－55℃
pH Stability pH 6.0－9.0 (25℃, 64hr)
Thermal stability below 50℃ (pH 7.3, 1hr)

Used in In Vitro Diagnostic tests and Life Sciences, Research & Development