Appearance: White powder
Activity: 100U/mg powder or more
Contaminants:
Lactate dehydrogenase ≤ 10-2%
Myokinase ≤ 5×10-2%
Pyruvate kinase ≤10-2%
Aldolase ≤ 2×10-2%
Carboxylase ≤ 5.0×10-2%
Phosphoglycerate kinase ≤ 5.0×10-2%
Stability: Store at -20°C
Molecular weight: 141,000
Isoelectric point: 5.6
Michaelis constant: 1.3×10-2 M (ethanol, pH 7.0)
Inhibitors: Ag+, Hg2+, SH-reagents
Optimum pH: 9.0
Optimum temperature: 25 – 35 °C
pH Stability: pH 6.0 – 9.0
Thermal stability: below 50 °C
Used in In Vitro Diagnostic tests and Life Sciences, Research & Development
Alcohol + NAD+ —> Aldehyde or ketone + NADH + H+ Alcohol + NAD+ <— Aldehyde or ketone + NADH + H+
< -20ºC