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Glycerol-3-Phosphate Oxidase (CM, REC)

L-α-Glicerol-3-fosfato : oxigênio oxidoredutase - EC 1.1.3.21

Product Description:

Molecular Weight: 75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G150)
Isoeletric Point: ~4.2 (230 000 Da (Sephadex G 200), 4 x 58 000 Da (SDS-gel electrophoresis))
Michaelis Constan (L-glycerol phosphate): K-phosphate buffer, 0.1 mol/L; pH 7.5: 1.36 x 10-2 mol/L (ensaio o-dianisidine)
Tris 0.1 mol/L; pH 7.6: 2.90 x 10-3 mol/L (o-dianisidine assay)
Tris 0.1 mol/L; pH 8.1: 1.40 x 10-3 mol/L (PAP assay)
Structure: Monomeric with FAD as cofactor
Inhibitors: Ag, Hg salts and SDS
pH optimum: 8.0-8.5

Application:

Used in diagnostic tests for the determination of glycerol or triglycerides in reaction coupled with Glycerol Kinase. Also used in specific applications in life sciences.

Storage:

< -20ºC

Presentations:

5 KU

50 KU

Custom*
> 500 KU

Custom*: Choose the desired quantity in a personalized way, as long as it is above 500 KU. Example: 548 KU.

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