Appearance : Light blue amorphous powder, lyophilized
Activity : GradeⅢ　200U/mg-solid or more (containing approx. 70% of stabilizers)
Contaminants : Catalase ≤1.0×10－1 % Phosphatase ≤2.0×10－2 %
Stabilizers : Bovine serum albumin (BSA), borax, basic amino acids.
Stability : Store at －20℃ (A decrease in activity of ca.20% may occur at 5℃ within 6 months.)
Molecular weight : Approx. 140,000
Isoelectric point : between 6.0 and 7.8.8 2
Michaelis constant : 2.5×10－4 M (Ascorbate)
Structure : 8 copper atoms per mol of enzyme 1,2
Inhibitors : cyanide, Na2S, diethyldithiocarbamate (Na)
Optimum pH : 5.6
Optimum temperature : approx. 30℃
pH Stability : pH 7.0－10.0 (25℃, 17hr)
Thermal stability : below 40℃ (pH 8.0, 30min)
Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.